{"id":46627,"date":"2020-08-17T17:59:55","date_gmt":"2020-08-17T17:59:55","guid":{"rendered":"https:\/\/lnbr.cnpem.br\/?page_id=46627"},"modified":"2024-03-22T14:28:50","modified_gmt":"2024-03-22T14:28:50","slug":"metabolomics","status":"publish","type":"page","link":"https:\/\/lnbr.cnpem.br\/en\/facilities\/metabolomics\/","title":{"rendered":"LNBR | Metabolomics"},"content":{"rendered":"<section class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;3\/4&#8243;][vc_row_inner][vc_column_inner][vc_column_text]<\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\"><strong>Metabolomics<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\">The Metabolomics Facility allows the identification and quantification of metabolites in complex biological samples by mass spectrometry coupled with liquid and gas chromatography. The metabolomics represents the chemical composition of a cell and allows an overview of cell functions, since it directly reflects its physiological state, allowing the characterization and differentiation of organisms. The facility serves users who work with bioenergy and green chemistry.<\/p>\n<p style=\"text-align: justify;\"><strong>Main skills and experimental techniques:<\/strong><\/p>\n<ul>\n<li style=\"text-align: justify;\">Identification and quantification analysis of primary metabolites and compounds of low molecular mass and low polarity;<\/li>\n<li style=\"text-align: justify;\">Identification, structural determination and quantification of secondary metabolite molecules, carbohydrates, lipids, among others.<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">The facility consists of two mass spectrometers coupled with liquid or gas chromatography. The GC-MS\/MS system has a triple quadrupole mass analyzer, QqQ(7010c, Agilent), designed for the analysis and quantification of volatile metabolites. The LC-MS\/MS system consists of a quadrupole followed by time-of-flight analyzer, Q-TOF (Synapt XS, Waters), used for the identification, structural determination and quantification of medium\/low molecular mass molecules (secondary metabolites, carbohydrates, lipids, among others).<\/p>\n<p style=\"text-align: justify;\"><strong>Start of operation:<\/strong>\u00a0in 2013 and 2015 for external users.<\/p>\n<p>[\/vc_column_text][vc_message]<\/p>\n<p style=\"text-align: justify;\"><strong>Mandatory citation to be used in user publications<\/strong><\/p>\n<p style=\"text-align: justify;\">This research used facilities of the Brazilian Biorenewables National Laboratory (LNBR), part of the Brazilian Center for Research in Energy and Materials (CNPEM), a private non-profit organization under the supervision of the Brazilian Ministry for Science, Technology, and Innovations (MCTI). The (FACILITY NAME) staff is acknowledged for the assistance during the experiments (proposal numbers).<\/p>\n<p>[\/vc_message][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_column_text]<\/p>\n<h3>Submission and usage period<\/h3>\n<table style=\"height: 197px;\" width=\"893\">\n<tbody>\n<tr>\n<td width=\"192\">\n<p style=\"text-align: center;\"><strong>Facilities<\/strong><\/p>\n<\/td>\n<td style=\"text-align: center;\" width=\"192\">\n<p style=\"text-align: center;\"><strong>Submission period 2024<\/strong><\/p>\n<\/td>\n<td style=\"text-align: center;\" width=\"192\">\n<p style=\"text-align: center;\"><strong>Performance\/usage period 2024<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"192\"><strong>Biophysics of Macromolecules\u00a0<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"192\">08\/Jan to 29\/Nov<\/td>\n<td style=\"text-align: center;\" width=\"192\">23\/Jan to 13\/Dec<\/td>\n<\/tr>\n<tr>\n<td width=\"192\"><strong>High-Performance Sequencing<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"192\">03\/Jan to 29\/Nov<\/td>\n<td style=\"text-align: center;\" width=\"192\">01\/Feb to 13\/Dec<\/td>\n<\/tr>\n<tr>\n<td width=\"192\"><strong>Metabolomics\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"192\">08\/Jan to 31\/Oct<\/td>\n<td style=\"text-align: center;\" width=\"192\">08\/Jan to 13\/Dec<\/td>\n<\/tr>\n<tr>\n<td width=\"192\"><strong>Bioprocess Development and Scale-up<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"192\"><strong>FOR 2024:<\/strong> 22\/Nov\/23 to 22\/Dec\/23<\/td>\n<td style=\"text-align: center;\" width=\"192\"><strong>FOR 2024:<\/strong> 2024<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column width=&#8221;1\/4&#8243;][vc_accordion active_tab=&#8221;1&#8243; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;USAGE &amp; SUBMISSION DATES&#8221;][vc_column_text]<strong>Submission and usage period<br \/>\n<\/strong>See below<\/p>\n<p><a href=\"https:\/\/sauonline.cnpem.br\/duo\/\" target=\"_blank\" rel=\"noopener noreferrer\"><b>Proposal submissions<\/b><\/a><\/p>\n<p><b><\/b><strong>User Support Service (SAU)<br \/>\n<\/strong><a href=\"mailto:sau@cnpem.br\">sau@cnpem.br<\/a>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;1&#8243; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;OPEN-ACCESS FACILITIES&#8221;][vc_column_text]<a href=\"https:\/\/lnbr.cnpem.br\/en\/facilities\/bioprocess-development-scale-up\/\">Bioprocess Development and Scale-up<\/a><br \/>\n<a href=\"https:\/\/lnbr.cnpem.br\/facilities\/dna-sequencing\/\">High-Performance Sequencing<\/a><br \/>\n<a href=\"https:\/\/lnbr.cnpem.br\/facilities\/metabolomics\/\">Metabolomics<\/a><br \/>\n<a href=\"https:\/\/lnbr.cnpem.br\/facilities\/macromolecules\/\">Biophysics of Macromolecules<\/a>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;false&#8221; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;USERS&#8221;][vc_custom_heading text=&#8221;Open-access Facilities&#8221; font_container=&#8221;tag:p|text_align:left&#8221; google_fonts=&#8221;font_family:Open%20Sans%3A300%2C300italic%2Cregular%2Citalic%2C600%2C600italic%2C700%2C700italic%2C800%2C800italic|font_style:400%20regular%3A400%3Anormal&#8221; link=&#8221;url:https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Ffacilities%2F|title:LNBR%20%7C%20Open-access%20facilities||&#8221;][vc_custom_heading text=&#8221;Training Opportunities&#8221; font_container=&#8221;tag:p|text_align:left&#8221; google_fonts=&#8221;font_family:Open%20Sans%3A300%2C300italic%2Cregular%2Citalic%2C600%2C600italic%2C700%2C700italic%2C800%2C800italic|font_style:400%20regular%3A400%3Anormal&#8221; link=&#8221;url:https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Ftraining-opportunities%2F|title:LNBR%20%7C%20Training%20opportunities||&#8221;][vc_custom_heading text=&#8221;Workshops &amp; Conferences&#8221; font_container=&#8221;tag:p|text_align:left&#8221; google_fonts=&#8221;font_family:Open%20Sans%3A300%2C300italic%2Cregular%2Citalic%2C600%2C600italic%2C700%2C700italic%2C800%2C800italic|font_style:400%20regular%3A400%3Anormal&#8221; link=&#8221;url:https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Fworkshops-conferences%2F|title:LNBR%20%7C%20Workshops%20%26%20Conferences||&#8221;][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;false&#8221; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;CONTACT US&#8221;][vc_column_text]<strong>Facility Coordination<br \/>\n<\/strong>Felipe Jun Fuzita<br \/>\n<a href=\"mailto:metabolomica@lnbr.cnpem.br\">metabolomica@lnbr.cnpem.br<\/a><\/p>\n<div id=\"gtx-trans\" style=\"position: absolute; left: 32px; top: 6px;\">\n<div class=\"gtx-trans-icon\"><\/div>\n<\/div>\n<p>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;false&#8221; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;SHARE&#8221;][vc_column_text]<\/p>\n<div class=\"single-share-box\">\n<div class=\"share-buttons\"><a class=\"linkedin\" title=\"LinkedIn\" href=\"https:\/\/www.linkedin.com\/shareArticle?mini=true&amp;url=https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F&amp;title=Lideran%C3%A7a&amp;summary=&amp;source=LNBR\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"social-text\">Share on LinkedIn<\/span><span class=\"screen-reader-text\">Share on LinkedIn<\/span><\/a><br \/>\n<a class=\"facebook\" title=\"Facebook\" href=\"http:\/\/www.facebook.com\/sharer.php?u=https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F&amp;t=Lideran%C3%A7a\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"social-text\">Share on Facebook<\/span><span class=\"screen-reader-text\">Share on Facebook<\/span><\/a><br \/>\n<a class=\"twitter\" title=\"Twitter\" href=\"https:\/\/twitter.com\/share?url=https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F&amp;text=Lideran%C3%A7a\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"social-text\">Tweet<\/span><span class=\"screen-reader-text\">Share on Twitter<\/span><\/a><br \/>\n<a class=\"whatsapp\" title=\"WhatsApp\" href=\"https:\/\/api.whatsapp.com\/send?text=Lideran%C3%A7a%20-%20https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F\" target=\"_blank\" rel=\"noopener noreferrer\" data-action=\"share\/whatsapp\/share\"><span class=\"social-text\">Share on WhatsApp<\/span><span class=\"screen-reader-text\">Share on WhatsApp<\/span><\/a><\/div>\n<p><!-- #content -->[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[\/vc_column][\/vc_row]<\/p>\n<\/div>\n<\/section>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;3\/4&#8243;][vc_row_inner][vc_column_inner][vc_column_text] Metabolomics The Metabolomics Facility allows the identification and quantification of metabolites in complex biological samples by mass spectrometry coupled with liquid and gas chromatography. The metabolomics represents the chemical composition of a cell and allows an overview of cell functions, since it directly reflects its physiological state, allowing the characterization and differentiation of&hellip;<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":46611,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-46627","page","type-page","status-publish","hentry","description-off"],"acf":[],"_links":{"self":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/46627","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/comments?post=46627"}],"version-history":[{"count":0,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/46627\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/46611"}],"wp:attachment":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/media?parent=46627"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}