{"id":46628,"date":"2020-08-17T17:59:55","date_gmt":"2020-08-17T17:59:55","guid":{"rendered":"https:\/\/lnbr.cnpem.br\/?page_id=46628"},"modified":"2026-10-07T17:24:44","modified_gmt":"2026-10-07T17:24:44","slug":"macromolecules","status":"publish","type":"page","link":"https:\/\/lnbr.cnpem.br\/en\/facilities\/macromolecules\/","title":{"rendered":"LNBR | HT-Biosys (High Throughput Biological Systems)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;3\/4&#8243;][vc_row_inner][vc_column_inner][vc_column_text css=&#8221;&#8221;]<\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\"><strong>HT-Biosys (High Throughput Biological Systems)<\/strong><\/span><\/p>\n<p data-pm-slice=\"1 1 []\">It provides an automated, high-throughput platform dedicated to the study of microorganisms and macromolecules. Its scope ranges from microorganism screening to advanced macromolecule analysis, making it particularly suitable for biotechnology applications such as metabolic engineering and enzyme engineering. The facility stands out for its high reproducibility, efficiency, and speed in generating experimental results.<\/p>\n<h3>Main skills and experimental techniques:<\/h3>\n<p>For Microorganisms:<\/p>\n<ul data-spread=\"false\">\n<li><strong>Hamilton Microlab STAR Liquid Handler<\/strong>, an automated liquid handling platform;<\/li>\n<li><strong>Growth Profiler 960 Enzyscreen<\/strong>, an incubation and orbital shaking system with image capture, designed for the simultaneous monitoring of up to 960 microbial cultures in 10 microplates;<\/li>\n<li><strong>BioLector XT Microbioreactor<\/strong>, a high-throughput microbioreactor designed for microbial, fungal, and algal cultivation in volumes of up to 2 mL;<\/li>\n<li><strong>BD Accuri C6 Plus and BD FACS Melody<\/strong>, flow cytometers;<\/li>\n<li><strong>Tecan Spark Spectrofluorimeter<\/strong>, a multimode microplate and cuvette reader that operates as both a spectrophotometer and fluorimeter.<\/li>\n<\/ul>\n<p>For Macromolecules:<\/p>\n<ul data-spread=\"false\">\n<li><strong>Hamilton Microlab STAR Liquid Handler<\/strong>, an automated liquid handling platform;<\/li>\n<li><strong>Tecan Spark Spectrofluorimeter<\/strong>, a multimode microplate and cuvette reader that operates as both a spectrophotometer and fluorimeter;<\/li>\n<li><strong>Malvern PEAQ-Auto ITC<\/strong>, an isothermal titration calorimeter that enables the accurate and direct characterization of biomolecular interactions in solution;<\/li>\n<li><strong>Agilent-Wyatt FFF-SEC-MALS<\/strong>, a hybrid system that combines separation techniques based on size exclusion chromatography (SEC) or field-flow fractionation (FFF) with multi-angle static light scattering (MALS). It enables the accurate characterization of the molar mass, size, conformation, and charge of macromolecules in solution.<\/li>\n<\/ul>\n<p><strong>Start of operation:<\/strong> in 2014 and 2015 for external users.<\/p>\n<p>As of 2026, the <strong>Macromolecular Biophysics<\/strong> facility has been renamed <strong>HT-Biosys (High Throughput Biological Systems)<\/strong>.[\/vc_column_text][vc_message]<\/p>\n<p style=\"text-align: justify;\"><strong>Mandatory citation to be used in user publications<\/strong><\/p>\n<p style=\"text-align: justify;\">This research used facilities of the Brazilian Biorenewables National Laboratory (LNBR), part of the Brazilian Center for Research in Energy and Materials (CNPEM), a private non-profit organization under the supervision of the Brazilian Ministry for Science, Technology, and Innovations (MCTI). The (FACILITY NAME) staff is acknowledged for the assistance during the experiments (proposal numbers).<\/p>\n<p>[\/vc_message][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3>Submission and usage period<\/h3>\n<table style=\"height: 197px;\" width=\"893\">\n<tbody>\n<tr>\n<td width=\"192\">\n<p style=\"text-align: center;\"><strong>Facilities<\/strong><\/p>\n<\/td>\n<td style=\"text-align: center;\" width=\"192\">\n<p style=\"text-align: center;\"><strong>Submission period 2025<\/strong><\/p>\n<\/td>\n<td style=\"text-align: center;\" width=\"192\">\n<p style=\"text-align: center;\"><strong>Performance\/usage period 2025<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"192\"><strong>Bioprocess Development and Scale-up<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"192\">10\/Nov\/25 to 19\/Dec\/25<\/td>\n<td style=\"text-align: center;\" width=\"192\">March to December\/2026<\/td>\n<\/tr>\n<tr>\n<td width=\"192\"><strong>Multi-omics<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"192\">1st call 05\/Jan\/2026 to 31\/Mar\/2026<br \/>\n2nd call 01\/Jul\/2026 to 30\/Sep\/2026<\/td>\n<td style=\"text-align: center;\" width=\"192\">1st call 01\/Jul\/2025 to 18\/Dez\/2026<br \/>\n2nd call 04\/Jan\/2027 to 30\/Jun\/2027<\/td>\n<\/tr>\n<tr>\n<td width=\"192\"><strong>HT-Biosys (High Throughput Biological Systems)<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"192\">19\/Jan\/2026 to 27\/Nov\/2026<\/td>\n<td style=\"text-align: center;\" width=\"192\">26\/Jan\/2026 to 11\/Dec\/2026<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column width=&#8221;1\/4&#8243;][vc_accordion active_tab=&#8221;1&#8243; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;USAGE &amp; SUBMISSION DATES&#8221;][vc_column_text]<strong>Submission and usage period<br \/>\n<\/strong>See below<\/p>\n<p><a href=\"https:\/\/sauonline.cnpem.br\/duo\/\" target=\"_blank\" rel=\"noopener noreferrer\"><b>Proposal submissions<\/b><\/a><\/p>\n<p><b><\/b><strong>User&#8217;s Office (EdU)<br \/>\n<\/strong><a href=\"mailto:edu@cnpem.br\">edu@cnpem.br<\/a>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;1&#8243; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;OPEN-ACCESS FACILITIES&#8221;][vc_column_text]<span class=\"--l sentence_highlight\"><a href=\"https:\/\/lnbr.cnpem.br\/en\/facilities\/bioprocess-development-scale-up\/\">Bioprocess Development and Scale-up<\/a><br \/>\n<a href=\"https:\/\/lnbr.cnpem.br\/en\/facilities\/multi-omicas\/\">Multi-omics<\/a><br \/>\n<\/span><a href=\"https:\/\/lnbr.cnpem.br\/en\/facilities\/macromolecules\/\"><span class=\"--l --r sentence_highlight\">HT-Biosys (High Throughput Biological Systems)<\/span><\/a>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;false&#8221; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;USERS&#8221;][vc_custom_heading text=&#8221;Open-access Facilities&#8221; font_container=&#8221;tag:p|text_align:left&#8221; google_fonts=&#8221;font_family:Open%20Sans%3A300%2C300italic%2Cregular%2Citalic%2C600%2C600italic%2C700%2C700italic%2C800%2C800italic|font_style:400%20regular%3A400%3Anormal&#8221; link=&#8221;url:https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Ffacilities%2F|title:LNBR%20%7C%20Open-access%20facilities||&#8221;][vc_custom_heading text=&#8221;Training Opportunities&#8221; font_container=&#8221;tag:p|text_align:left&#8221; google_fonts=&#8221;font_family:Open%20Sans%3A300%2C300italic%2Cregular%2Citalic%2C600%2C600italic%2C700%2C700italic%2C800%2C800italic|font_style:400%20regular%3A400%3Anormal&#8221; link=&#8221;url:https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Ftraining-opportunities%2F|title:LNBR%20%7C%20Training%20opportunities||&#8221;][vc_custom_heading text=&#8221;Workshops &amp; Conferences&#8221; font_container=&#8221;tag:p|text_align:left&#8221; google_fonts=&#8221;font_family:Open%20Sans%3A300%2C300italic%2Cregular%2Citalic%2C600%2C600italic%2C700%2C700italic%2C800%2C800italic|font_style:400%20regular%3A400%3Anormal&#8221; link=&#8221;url:https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Fworkshops-conferences%2F|title:LNBR%20%7C%20Workshops%20%26%20conferences||&#8221;][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;false&#8221; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;CONTACT US&#8221;][vc_column_text]<strong>Facility Coordination<br \/>\n<\/strong>Mariane Domingues<br \/>\n<a href=\"mailto:htbiosys@lnbr.cnpem.br\">htbiosys@lnbr.cnpem.br<\/a>[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[vc_accordion active_tab=&#8221;false&#8221; collapsible=&#8221;yes&#8221; style=&#8221;2&#8243;][vc_accordion_tab title=&#8221;SHARE&#8221;][vc_column_text]<\/p>\n<div class=\"single-share-box\">\n<div class=\"share-buttons\"><a class=\"linkedin\" title=\"LinkedIn\" href=\"https:\/\/www.linkedin.com\/shareArticle?mini=true&amp;url=https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F&amp;title=Lideran%C3%A7a&amp;summary=&amp;source=LNBR\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"social-text\">Share on LinkedIn<\/span><span class=\"screen-reader-text\">Share on LinkedIn<\/span><\/a><br \/>\n<a class=\"facebook\" title=\"Facebook\" href=\"http:\/\/www.facebook.com\/sharer.php?u=https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F&amp;t=Lideran%C3%A7a\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"social-text\">Share on Facebook<\/span><span class=\"screen-reader-text\">Share on Facebook<\/span><\/a><br \/>\n<a class=\"twitter\" title=\"Twitter\" href=\"https:\/\/twitter.com\/share?url=https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F&amp;text=Lideran%C3%A7a\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"social-text\">Tweet<\/span><span class=\"screen-reader-text\">Share on Twitter<\/span><\/a><br \/>\n<a class=\"whatsapp\" title=\"WhatsApp\" href=\"https:\/\/api.whatsapp.com\/send?text=Lideran%C3%A7a%20-%20https%3A%2F%2Fpages.cnpem.br%2Flnbrteste%2Flideranca%2F\" target=\"_blank\" rel=\"noopener noreferrer\" data-action=\"share\/whatsapp\/share\"><span class=\"social-text\">Share on WhatsApp<\/span><span class=\"screen-reader-text\">Share on WhatsApp<\/span><\/a><\/div>\n<p><!-- #content -->[\/vc_column_text][\/vc_accordion_tab][\/vc_accordion]<div class=\"hr-thin style-line\" style=\"width: 100%;border-color: #606060;border-top-width: 5px;\"><\/div>[\/vc_column][\/vc_row]<\/p>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;3\/4&#8243;][vc_row_inner][vc_column_inner][vc_column_text css=&#8221;&#8221;] HT-Biosys (High Throughput Biological Systems) It provides an automated, high-throughput platform dedicated to the study of microorganisms and macromolecules. Its scope ranges from microorganism screening to advanced macromolecule analysis, making it particularly suitable for biotechnology applications such as metabolic engineering and enzyme engineering. The facility stands out for its high reproducibility, efficiency,&hellip;<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":46611,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-46628","page","type-page","status-publish","hentry","description-off"],"acf":[],"_links":{"self":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/46628","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/comments?post=46628"}],"version-history":[{"count":2,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/46628\/revisions"}],"predecessor-version":[{"id":49805,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/46628\/revisions\/49805"}],"up":[{"embeddable":true,"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/46611"}],"wp:attachment":[{"href":"https:\/\/lnbr.cnpem.br\/en\/wp-json\/wp\/v2\/media?parent=46628"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}