DNA Sequencing

The Next Generation Sequencing (NGS) facility is dedicated to large scale sequencing of nucleic acids (DNA/RNA), enabling a diversified range of studies from gene expression analysis of samples from several biological systems, to the sequencing of genomes, metagenomes, metatranscriptomes, and target sequencing of molecular markers such as 16S rDNA and ITS).

Main skills and experimental techniques:

  • Sequencing of Metagenomes and Metatranscriptomes from complex environmental samples.
  • Target sequencing of molecular markers such as 16S rDNA for Prokaryotic and ITS for eukaryotes diversity analysis
  • Sequencing of genomes and transcriptomes (Stranded RNA-seq) from diverse organisms such as filamentous fungi, bacteria, yeasts, and plants.

Currently, the facility is composed of two second-generation technology equipment, a MiSeq and a HiSeq 2500, both from the Illumina Platform (Illumina Inc.). Furthermore, a highly qualified team is dedicated to assist and advise users during all activities, including the experimental design, sample preparation, and sequencing.

Start of operation: in 2014 and 2015 for external users.

Mandatory citation to be used in user publications

The user must explicitly declare the infrastructure used in LNBR, according to the specific guidelines of each facility disclosed on the site, in any article, thesis, or other published material that uses data obtained in the realization of its proposal. Users must also cooperate in providing this information when requested.

SOME OF THIS WORK WAS PERFORMED AT THE BRAZILIAN BIORENEWABLES NATIONAL LABORATORY (LNBR/CNPEM/MCTI) DURING THE USE OF THE [FACILITY NAME] OPEN ACCESS FACILITY